A Brief Introduction of Pipets- Modes and Types


A pipet is a laboratory equipment used to measure and transfer small quantities of liquid, usually milliliters (mL) or microliters from one container to another. Due to its transferring property, the transfer pipets are widely used in chemistry, medical sciences, molecular biology, and various experiments. The design of the pipet completely fulfills its efficiency in the working environment.

Modes of Pipet

Majorly, there are two modes of pipet used in the laboratories:

  • Forward mode
  • Reverse mode

Forward Mode

Forward mode is used for most aqueous solutions because it is more accurate and precise when it comes to providing better outcomes of laboratory work.

In forward mode pipet, firstly, the plunger is pressed to the second stop by immersing the pipet tip in the sample. And then, the plunger is released to grasp the solution from the container. Finally, the complete solution is dispersed to its destination by pressing the plunger to the second stop.

Reverse Mode

The reverse mode of transfer pipets is always recommended for a viscous solution because the forward mode is not up-to-mark for viscous solution resulting in under-delivery of the result. Before immersing in the solution, the plunger is pressed to the second stop to grasp the solution properly. Finally, it is depressing to the first stop to transfer the sample from the source container to the destination.

Types of Pipets

As there are many pipets available on the market to buy, do not forget that some pipets offer more accuracy than others. Here we have listed some of the types of pipets:

Volumetric Pipet

Generally, volumetric pipet is widely used in schools, educational institutions, and professional laboratories to examine chemical properties and reactions. It is globally famous for its accuracy and can measure till significant figures. They are available in various sizes enabling researchers to measure the solution.

Micro Pipet

Micro pipet allows scientists and researchers to get a very accurate reading for their experiments. Micro pipet can be calibrated at least once every 3 to 6 months.

Graduated Pipet  

These transfer pipets are less accurate compared to volumetric pipets. It is of two types:

Mohr Graduated Pipet: Mohr graduated pipet which is also known as “drain out pipet.” A “zero” is marked at the starting of the conical end of Mohr graduated pipet

Serological Graduated Pipet: “Blow out pipet” is another name of the serological pipet. Unlike Mohr graduated pipet, zero is not marked at the start of its conical end.

Pasteur Pipet  

Pasteur pipet found its name from a French physician Louis Pasteur. When it comes to measurement, Pasteur pipets are the most inaccurate. It is made of glass with a bulb-shaped top. It is neither graduated nor calibrated. And these types of pipets are generally used in biology labs rather than in chemical labs. 

Vacuum-Assisted Pipets  

There are two types of vacuum-assisted pipets-

Graduated vacuum-assisted pipets: They utilize several graduation marks.

Volumetric vacuum-assisted pipets: They estimate a single volume. Thus, displays only one graduation mark. They don’t contain pistons, that’s why they require a suction device.

 Pipetting demands concentration and accuracy. With appropriate training, and practice, and by using regularly calibrated pipets, you can always deliver accurate results.

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