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Western blotting is a method for identifying a specific protein with the help of blotting paper in a sample that contains other proteins. Each western blot experiment uses a specific antibody against a particular protein. These antibodies attach to the target protein and aid in its later quantification. The study method known as western blotting is widely used in molecular biology, immunology, and allied sciences. Western blotting is essential for students who want to work in these fields. However, novice students learning about it could find the lessons frightening. Fortunately, there are numerous approaches to making it more interesting and approachable. Here are five suggestions for educators to make the subject more engaging based on students' challenges when learning about Western blotting. Each item of guidance addresses a specific issue that students encounter. Explore Real-World Applications HIV-AIDS diagnosis can be made via Western blotting. The method is specifically us...

Labs use electrophoresis to examine medicines, vaccinations, forensics, DNA profiling, or other life science applications. The method is also employed in fields like food science and mining with the help of electrophoresis accessories . In gel electrophoresis, proteins or nucleic acids move across a porous gel matrix. Proteins and nucleic acids both have a net-negative electrical charge, which is used to help the target molecule move more easily across the medium. Proteins and nucleic acids can be separated using gel electrophoresis according to their size. Horizontal Gel Electrophoresis During horizontal gel electrophoresis, a horizontally cast gel is immersed in a running buffer inside the gel box. The ionic running buffer creates a charge gradient when a current is delivered. A layer of agarose gel divides the two chambers in the gel box. A cathode is situated at one end, and an anode at the other. The gel, which warms up as a charge is applied, is cooled by the buffer. The running ...